The concept, application discipline, structure, advantages and disadvantages of atomic force microscope

Chinese name:

atomic force microscope

English name:

atomic force microscope

definition:

A microscope designed to take three-dimensional images at high speed based on the principle of a scanning tunnel microscope. Can observe the changes in the activities of large molecules in the body.

Applied discipline:

Cell biology (first-level discipline); cell biology technology (second-level discipline)

Atomic Force Microscope (AFM), an analytical instrument that can be used to study the surface structure of solid materials including insulators. It studies the surface structure and properties of substances by detecting the extremely weak interatomic interaction force between the surface of the sample to be tested and a miniature force-sensitive element. Fix a pair of micro-cantilevers that are extremely sensitive to weak forces, and the tiny tip of the other end is close to the sample. At this time, it will interact with it, and the force will cause the micro-cantilever to deform or change its motion state. When scanning the sample, using sensors to detect these changes, you can obtain the force distribution information, so as to obtain surface structure information with nanometer resolution.

advantage

Image observed by atomic force microscope

Image observed by atomic force microscope

Compared with the scanning electron microscope, the atomic force microscope has many advantages. Unlike electron microscopes that can only provide two-dimensional images, AFM provides true three-dimensional surface maps. At the same time, AFM does not require any special treatment of the sample, such as copper or carbon, which will cause irreversible damage to the sample. Third, the electron microscope needs to operate under high vacuum conditions, and the atomic force microscope can work well under normal pressure and even in a liquid environment. This can be used to study biological macromolecules and even living biological tissues.

Disadvantages

Compared with scanning electron microscope (SEM), the disadvantage of AFM is that the imaging range is too small, the speed is slow, and it is too affected by the probe. Atomic Force Microscope (Atomic Force Microscope) is a new type of instrument with high resolution at the atomic level invented after Scanning Tunneling Microscope. It can perform physical properties of nano-areas on various materials and samples in the atmosphere and liquid environment. Including the detection of morphology or direct nano manipulation; it has been widely used in the fields of semiconductor, nano-functional materials, biology, chemical industry, food, pharmaceutical research and research institutes in various nano-related disciplines, etc. Basic tools for research. Compared with scanning tunneling microscope, atomic force microscope has wider applicability because it can observe non-conductive samples. The scanning force microscope (Scanning Force Microscope) currently widely used in scientific research and industry is based on the atomic force microscope.

Instrument structure:

In the system of Atomic Force Microscopy (AFM), it can be divided into three parts: force detection part, position detection part, and feedback system.

Force detection section

In an atomic force microscope (AFM) system, the force to be detected is the van der Waals force between atoms. Therefore, in this system, a tiny cantilever is used to detect the change in force between atoms. The microcantilever is usually made of a silicon wafer or silicon nitride wafer that is generally 100 to 500 μm long and about 500 nm to 5 μm thick. There is a sharp needle tip at the top of the microcantilever, which is used to detect the sample-needle interaction force. This tiny cantilever has certain specifications, such as length, width, elastic coefficient, and shape of the needle tip, and the selection of these specifications is based on the characteristics of the sample and the different operating modes, and different types of probes are selected.

Position detection section

atomic force microscope

atomic force microscope

In the atomic force microscope (AFM) system, when the needle tip interacts with the sample, the cantilever cantilever will swing, so when the laser is irradiated on the end of the microcantilever, the position of the reflected light will also be caused by the cantilever swing There is a change, which causes an offset. In the entire system, the laser spot position detector is used to record the offset and convert it into an electrical signal for the SPM controller to perform signal processing.

Feedback system

In an atomic force microscope (AFM) system, after a signal is taken in through a laser detector, this signal is used as a feedback signal in the feedback system as an internal adjustment signal, and drives a scan usually made of piezoelectric ceramic tubes Appropriate movement of the instrument to maintain a certain force between the sample and the needle tip.

to sum up

The AFM system uses a scanner made of piezoelectric ceramic tubes to precisely control tiny scanning movements. Piezoelectric ceramic is a kind of material with peculiar performance. When voltage is applied to the two symmetrical end surfaces of piezoelectric ceramic, piezoelectric ceramic will expand or contract in a specific direction. The size of elongation or contraction has a linear relationship with the magnitude of the applied voltage. That is to say, the tiny expansion and contraction of the piezoelectric ceramic can be controlled by changing the voltage. Generally, three piezoelectric ceramic blocks representing X, Y, and Z directions are used to form the shape of a tripod. By controlling the expansion and contraction in the X and Y directions, the purpose of driving the probe to scan the sample surface is achieved; by controlling the expansion and contraction of the piezoelectric ceramics in the Z direction To achieve the purpose of controlling the distance between the probe and the sample.

Atomic Force Microscope (AFM) combines the above three parts to present the surface characteristics of the sample: In the AFM system, a tiny cantilever is used to sense the interaction between the tip and the sample, This force will make the micro cantilever swing, and then use the laser to illuminate the end of the cantilever. When the swing is formed, the position of the reflected light will change and cause an offset. At this time, the laser detector will record this offset. The signal at this time will also be given to the feedback system to facilitate the system to make appropriate adjustments, and finally the surface characteristics of the sample will be presented as an image.

Commodity

18mm Laminated Particleboard/Chipboard

Size

1220*2440/1250*2500mm,1220*3050mm, 1525*3050mm, 1220*5400mm

Glue

MR, WBP and Melamine,E1

Core

using poplar, combined, hardwood, Birch, pine etc.

face/back

Okume, Bintangor, poplar, birch, pine etc

Thickness

2.5 -40mm

Moisture

8-14%

Payment terms

T/T, LC at sight

Price Terms

EXW, FOB, CNF, CIF

Usage

can be widely used in construction, mould, etc.

Packing

standard export packing by Plywood or MDF, wrapped in plastic bag and then strengthened by iron straps

Laminated Particleboard

Laminated Particle Board,Pre Laminated Particle Board,Furniture Laminated Particle Board,Melamine Laminated Particle Board

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